Mr Wilson Gauci
Honours Student Graduate
Department of Physiology, Anatomy and Microbiology
La Trobe University
WilsGauc10@gmail.com
Research Activities
Attempted generation of an artificial res-site hunter transposon using restriction cloning techniques. Creation of theorised clone constructs in silico using Geneious Prime. Transposition assays of the Tn502 res-site hunter transposon to observe transposition frequencies in the res-site of IncP plasmids and its insertional orientation.Techniques/Expertise
Expertise- Bacterial transposons (res-site hunter transposons)
- Bacterial plasmids
- Molecular Cloning & DNA analysis
- Restriction/Conventional Cloning in E.coli
- Bacterial culturing
- Conjugative mating
- Transposition assays
- Plasmid extractions (Minipreps)
- Gel electrophoresis
- PCR and Colony PCR
- DNA sequence analysis & in silico manipulation using Geneious Prime
- PCR primer mapping
